Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers

نویسندگان

چکیده

Medicago polymorpha L. is a herbaceous legume that can be useful pasture crop, especially in Mediterranean climates. This study aimed to analyze the genetic variation five populations of M. collected from different eco- geographic regions Tunisia using eight SSR markers. The transferability 112 markers distributed on chromosomes truncatula Gaertn. showed 50 could amplified polymorpha. Among these markers, (8) were polymorphous. A high level polymorphism (126 polymorphic alleles with an average 5.3 per locus) and moderate diversity found all studied populations, observed expected heterozygosities averaging between 0 0.69, respectively. Results analysis molecular variance (AMOVA) revealed most was within (76%). Moderate levels population differentiation (FST = 0.12 0.19) accompanied by rate gene flow (Nm 1.08 1.83) recorded. (FST) not dependent geographical distances (r 0.395, p 0.524), suggesting are geographically isolated. Our results clustered into three groups. first group formed El Kef (TNP7) Bulla Regia (TNP9), second Enfidha (TNP1) third Soliman (TNP8) Mateur (TNP11). obtained our helpful for breeders considering introduction some lines this species breeding programs.

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ژورنال

عنوان ژورنال: Chilean Journal of Agricultural Research

سال: 2021

ISSN: ['0718-5820', '0718-5839']

DOI: https://doi.org/10.4067/s0718-58392021000100053